Area : HPLC Columns & Accessories
Blaze200™ High Temperature Silica Columns
Silica Columns for use at Extreme Temperatures
has developed a family of polycarbosilane bonded silica columns
that resist hydrolytic breakdown at temperatures up to 200ºC
under reverse phase conditions.
Although there are a variety
of stationary phases such as polymeric
and graphitic carbon materials that can be used at elevated temperatures,
most HPLC chemists prefer the selectivity and higher efficiencies
of silica-based columns. Increasing temperature can drastically
limit the column lifetime when the bonded phase is based on traditional
chemistries. Most silica-based HPLC columns can only be used to
temperatures up to 60°C and a pH range of 3-8. Such columns
have a propensity to degrade as water attacks the underlying support
new Blaze 200 ™ column overcomes these obstacles. It consists of
a new polydentate silica phase that is stable at extreme temperatures,
with added pH stability. The development of a new polycarbosilane
bonding chemistry that is hydrolytically stable against breakdown
in aqueous environments facilitates such analyses. It integrates
multiple point attachments to the silica with a high degree of cross-linking.
This combination creates a highly protective barrier over the silica
backbone, protecting it from hydrolytic attack.
offers traditional C18 selectivity, while providing enhanced temperature
stability for use up to 200°C and pH conditions as high as 12.
Columns based on this chemistry are useful for both isothermal and
temperature programmed separations over wide ranges of temperature.
Other columns that can take the heat:
The Thermo Electron
Hypercarb® column has proven durable under temperature programmed
conditions up to 200ºC. For more information on Hypercarb®
Further expanding the range of selectivity,
Jordi polymeric stationary phases have been used for temperature
programmed HPLC successfully to 150°C. For more information,
contact Jordi FLP at www.jordiassoc.com
When these columns from
other manufacturers are ordered for high temperature HPLC use,
all stainless steel hardware (including the column frits) must
be specified. Thermo Electron and Jordi both have special
part numbers for all stainless steel hardware. Please contact
them for more information.
Notes 804, 806, 808 and 811 for more information.
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