Main Products Area : HPLC
Advantages of Using High Temperatures in HPLC
The impact of
temperature on chromatographic performance for HPLC has been investigated
and the benefits of increasing the temperature are well documented in the
HPLC literature. The three major benefits are as follows:
Shorter run times result from lower viscosity
and decreased back pressure at elevated temperatures. As the temperature
increases, the viscosity of the mobile phase decreases. Since the mobile
phase can now move through the column bed more easily, the pressure
required to pump the mobile phase through the column is relaxed.
Cosequently, smaller particles can be used for higher plate counts, and
higher flow rates can be used without exceeding pump pressure limits.
2. Higher efficiency and better
Lower plate heights due to increased diffusion
rates improve the efficiency and resolution. The diffusion rate increases,
in both the mobile phase and within the stationary phase as the temperature
increases. The net result of increasing the temperature is a reduction in
plate height over an extended range of the mobile phase flow rate.
3. The ability to change
retention through temperature programming
third major advantage is the ability to replace solvent gradients with
temperature gradients. This can be accomplished by temperature programming.
Increasing the temperature can result in changes in selectivity and in the
mobile phase solvating strength.
- Elimination of solvent
- Greater apparent
efficiency of later eluting peaks
- Enables rapid elution of
compounds within a wide range of retention
- Same advantages as
programmed GC compared to isothermal runs
Please see Application Notes and Technical Notes for more
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