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Supercritical Fluid
Chromatography (SFC)

Series 4000 SFC
Series 4000 SFC Features
Series 4000 SFC Specs
SFC Columns & Accessories

Temperature Programmed HPLC

HPLC Overview
Caloratherm™ Mobile Phase    Temp Conditioner
Caloratherm™ Specs
Polaratherm™ Total
   Temperature Controller

Polaratherm™ Specs
Mobile Phase Preheater
HPLC Columns
   & Accessories

Selerity Secure-Fit™ Connector

Literature and Brochures

Series 4000 SFC Brochure
High Temperature HPLC Brochure
Application Notes
Technical Notes

 

 


Main Products Area : HPLC

Advantages of Using High Temperatures in HPLC

The impact of temperature on chromatographic performance for HPLC has been investigated and the benefits of increasing the temperature are well documented in the HPLC literature. The three major benefits are as follows:

1. Speed

Shorter run times result from lower viscosity and decreased back pressure at elevated temperatures. As the temperature increases, the viscosity of the mobile phase decreases. Since the mobile phase can now move through the column bed more easily, the pressure required to pump the mobile phase through the column is relaxed. Cosequently, smaller particles can be used for higher plate counts, and higher flow rates can be used without exceeding pump pressure limits.

2. Higher efficiency and better resolution

Lower plate heights due to increased diffusion rates improve the efficiency and resolution. The diffusion rate increases, in both the mobile phase and within the stationary phase as the temperature increases. The net result of increasing the temperature is a reduction in plate height over an extended range of the mobile phase flow rate.

3. The ability to change retention through temperature programming

The third major advantage is the ability to replace solvent gradients with temperature gradients. This can be accomplished by temperature programming. Increasing the temperature can result in changes in selectivity and in the mobile phase solvating strength.

Temperature Gradients

  • Elimination of solvent gradients
  • Greater apparent efficiency of later eluting peaks
  • Enables rapid elution of compounds within a wide range of retention
  • Same advantages as programmed GC compared to isothermal runs

Please see Application Notes and Technical Notes for more information.

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